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    首頁>>免疫學>>一抗>>活化半胱胺酸蛋白酶蛋白3抗體
    活化半胱胺酸蛋白酶蛋白3抗體
    • 產品貨號:
      BN41484R
    • 中文名稱:
      活化半胱胺酸蛋白酶蛋白3抗體
    • 英文名稱:
      Rabbit anti-caspase-3 p12 subunit Polyclonal antibody
    • 品牌:
      Biorigin
    • 貨號

      產品規格

      售價

      備注

    • BN41484R-50ul

      50ul

      ¥1486.00

      交叉反應:Mouse,Human(predicted:Rabbit,Dog,Rat) 推薦應用:WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

    • BN41484R-100ul

      100ul

      ¥2360.00

      交叉反應:Mouse,Human(predicted:Rabbit,Dog,Rat) 推薦應用:WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

    • BN41484R-200ul

      200ul

      ¥3490.00

      交叉反應:Mouse,Human(predicted:Rabbit,Dog,Rat) 推薦應用:WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

    產品描述

    英文名稱caspase-3 p12 subunit
    中文名稱活化半胱胺酸蛋白酶蛋白3抗體
    別    名Caspase-3 subunit p12; cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.  




    研究領域細胞生物  免疫學  信號轉導  細胞凋亡  
    抗體來源Rabbit
    克隆類型Polyclonal
    交叉反應Human, Mouse,  (predicted: Rat, Dog, Rabbit, )
    產品應用ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 (石蠟切片需做抗原修復)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量12/32kDa
    細胞定位細胞漿 
    性    狀Liquid
    濃    度1mg/ml
    免 疫 原KLH conjugated synthetic peptide derived from human caspase-3 p12 subunit:201-277/277 
    亞    型IgG
    純化方法affinity purified by Protein A
    儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    PubMedPubMed
    產品介紹The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

    Function:
    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

    Subunit:
    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.

    Subcellular Location:
    Cytoplasm.

    Tissue Specificity:
    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

    Post-translational modifications:
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

    Similarity:
    Belongs to the peptidase C14A family.

    SWISS:
    P42574

    Gene ID:
    836

    Database links:

    Entrez Gene: 836 Human

    Entrez Gene: 12367 Mouse

    Entrez Gene: 397244 Pig

    Entrez Gene: 100008840 Rabbit

    Entrez Gene: 25402 Rat

    Omim: 600636 Human

    SwissProt: P42574 Human

    SwissProt: P70677 Mouse

    SwissProt: Q95ND5 Pig

    SwissProt: Q8MJC3 Rabbit

    SwissProt: P55213 Rat

    Unigene: 141125 Human

    Unigene: 34405 Mouse

    Unigene: 10562 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

    正常情況下caspase-3以無活性酶原形式存在于細胞漿中。當 caspase-3被上游caspases激活生成活化的caspase-3 p17和p12亞基,對細胞凋亡的發生和調制發揮重要作用。

























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