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    磷酸化p21蛋白抗體
    • 產品貨號:
      BN41063R
    • 中文名稱:
      磷酸化p21蛋白抗體
    • 英文名稱:
      Rabbit anti-phospho-CDKN1A/p21 (Thr57) Polyclonal antibody
    • 品牌:
      Biorigin
    • 貨號

      產品規格

      售價

      備注

    • BN41063R-100ul

      100ul

      ¥2470.00

      交叉反應:Human,Mouse,Rat 推薦應用:IHC-P,IHC-F,IF,Flow-Cyt,ELISA

    產品描述

    英文名稱phospho-CDKN1A/p21 (Thr57)
    中文名稱磷酸化p21蛋白抗體
    別    名CDKN1A/p21 (phospho Thr57); p21 (phospho T57); p21 (phospho Thr57); Activating Fragment 1; CAP20; Cation chloride cotransporter-interacting protein 1; CDK Interacting Protein 1; CDK-interacting protein 1; CDKI; CDKN 1; CDKN1; CDKN1A; CIP1; Cyclin Dependent Kinase Inhibitor 1A; Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (P21); Cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA Synthesis Inhibitor; MDA 6; MDA-6; MDA6; Melanoma Differentiation Associated Protein 6; Melanoma differentiation-associated protein 6; Melanoma differentiation-associated protein; p21; P21 protein; p21CIP1; p21WAF; PIC1; SDI1; SLC12A9; WAF1; Wildtype p53 Activating Fragment 1; Wildtype p53-activated fragment 1; CDN1A_HUMAN.  
    產品類型磷酸化抗體 
    研究領域腫瘤  免疫學  信號轉導  細胞周期蛋白  
    抗體來源Rabbit
    克隆類型Polyclonal
    交叉反應Human, Mouse, Rat, 
    產品應用ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 (石蠟切片需做抗原修復)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量18kDa
    細胞定位細胞核 細胞漿 
    性    狀Liquid
    濃    度1mg/ml
    免 疫 原KLH conjugated Synthesised phosphopeptide derived from human CDKN1A around the phosphorylation site of Thr57:TE(p-T)PL 
    亞    型IgG
    純化方法affinity purified by Protein A
    儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    PubMedPubMed
    產品介紹This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

    Function:
    May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

    Subunit:
    Interacts with HDAC1; the interaction is prevented by competitive binding of C10orf90/FATS to HDAC1 facilitating acetylation and protein stabilization of CDKN1A/p21. Interacts with MKRN1. Interacts with PSMA3. Interacts with PCNA. Component of the ternary complex, cyclin D-CDK4-CDKN1A. Interacts (via its N-terminal domain) with CDK4; the interaction promotes the assembly of the cyclin D-CDK4 complex, its nuclear translocation and promotes the cyclin D-dependent enzyme activity of CDK4. Binding to CDK2 leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair. Interacts with PIM1.

    Subcellular Location:
    Cytoplasmic and Nuclear.

    Tissue Specificity:
    Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain.

    Post-translational modifications:
    Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability.
    Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
    Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1.

    Similarity:
    Belongs to the CDI family.

    SWISS:
    P38936

    Gene ID:
    1026

    Database links:

    Entrez Gene: 1026 Human

    Entrez Gene: 12575 Mouse

    Entrez Gene: 114851 Rat

    Omim: 116899 Human

    SwissProt: P38936 Human

    SwissProt: P39689 Mouse

    Unigene: 370771 Human

    Unigene: 195663 Mouse

    Unigene: 10089 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

    p21蛋白的過度表達與腫瘤的類型、惡性度、分期以及病人的預后密切相關。主要用于胃腸道癌腫、乳腺癌、肺癌等惡性腫瘤的研究。


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